p53 gene deletions in paraffin-preserved lymphoid tumors from irradiated mice.
Leukemia research
(2000)
24:511-517.
Abstract
Experiments were performed to measure deletions in the p53 gene in paraffin-embedded tissues (tumors and control) derived from mice exposed to gamma-rays or neutrons up to 28 years ago. Deletions in exons 1, 3, 4, 5, 6, 7 and 9 were monitored by PCR and Southern blotting techniques. The results of these experiments demonstrated p53 deletions in only 1/6 spontaneous tumors but in 5/6 gamma-ray-induced and 5/6 neutron-induced tumors. Exons deleted in tumors from gamma-ray exposed mice were similar to those deleted in tumors from neutron-exposed mice. They document differences in spectra of p53 deletions in comparing spontaneous radiation-induced tumors.
Experiments were performed to measure deletions in the p53 gene in paraffin-embedded tissues (tumors and control) derived from mice exposed to gamma-rays or neutrons up to 28 years ago. Deletions in exons 1, 3, 4, 5, 6, 7 and 9 were monitored by PCR and Southern blotting techniques. The results of these experiments demonstrated p53 deletions in only 1/6 spontaneous tumors but in 5/6 gamma-ray-induced and 5/6 neutron-induced tumors. Exons deleted in tumors from gamma-ray exposed mice were similar to those deleted in tumors from neutron-exposed mice. They document differences in spectra of p53 deletions in comparing spontaneous radiation-induced tumors.
MeSH terms
Paraffin Southern Blotting Lymphoma Inbred BALB C Mice Inbred C57BL Mice Gene Deletion Exons Tumor Suppressor Protein p53 Polymerase Chain Reaction Mice genetics
Paraffin Southern Blotting Lymphoma Inbred BALB C Mice Inbred C57BL Mice Gene Deletion Exons Tumor Suppressor Protein p53 Polymerase Chain Reaction Mice genetics
Note
Automated medline import. Electronic deposit: .Electronic publication: 2000-04-27. Publication Date: 2000-06-01. Publication status: ppublish. Status: MEDLINE.
Automated medline import. Electronic deposit: .Electronic publication: 2000-04-27. Publication Date: 2000-06-01. Publication status: ppublish. Status: MEDLINE.